《植物生理学报》 2017, 53(9): 1781-1787

NaCl胁迫下杂交一代番茄幼苗不同部位对蛭石引发的响应

霍文雨^1,2,*, 吴凌云^1,*, 姚东伟^1,*, 朱月林^2,*, 李明^1,**

¹上海市农业科学院设施园艺研究所, 上海市设施园艺技术重点实验室, 上海201403; ²南京农业大学园艺学院, 南京210095

通信作者：霍文雨；E-mail: yyl3@saas.sh.cn

摘　要：

引发作为种子播前处理技术, 可以提高逆境条件下种子活力。本文探讨了盐胁迫下杂交一代番茄2个品种(‘大红合作909’和‘粉红合作906’)幼苗不同部位对蛭石引发的响应。利用蛭石在15°C黑暗条件下对2个番茄品种的种子引发处理5 d, 引发和未引发的种子分别放入浓度为0、100 mmol·L^-1 NaCl盐溶液的发芽盒内, (25±1)°C发芽10 d后取根、茎、叶进行各项生理指标的测定。研究显示, 与未引发种子相比, 蛭石引发能显著提高幼苗茎、根干重和鲜重。此外, 蛭石引发能显著降低盐胁迫下茎、根中丙二醛含量, 提高根、茎脯氨酸含量、抗氧化酶活性(SOD、POD、CAT), 积累较多渗透调节物质(游离氨基酸、可溶性蛋白、可溶性糖), 提高了叶绿素含量; 且发现叶绿素b (Chlb)比叶绿素a (Chla), 类胡萝卜素(carotenoid, Car)比叶绿素(a+b)在盐胁迫下稳定。结果表明: 蛭石引发能够提高盐胁迫下番茄幼苗不同器官抗氧化酶活性, 促进渗透调节物质的合成, 降低膜透性和膜脂过氧化水平, 保护光合器官及细胞膜结构的稳定性, 从而提高番茄幼苗的耐盐能力。

关键词：番茄幼苗; 蛭石引发; 光合色素; 盐胁迫

收稿：2017-07-03   修定：2017-08-25

资助：上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-5号]。

Response of different organs in tomato hybrid seedlings to solid matrix priming under NaCl stress

HUO Wen-Yu^1,2,*, WU Ling-Yun^1,*, YAO Dong-Wei^1,*, ZHU Yue-Lin^2,*, LI Ming^1,**

¹Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; ²College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: HUO Wen-Yu; E-mail: yyl3@saas.sh.cn

Abstract:

Priming can improve seed vigor under stress conditions as a seed sowing treatment technique. This study investigated the responses of different organs of two tomato (Lycopersicon esculentum M.) hybrid seedlings (‘Red cooperation 909’ and ‘Pink cooperation 906’) to solid matrix priming under salt stress. The seeds of two tomato varieties were primed with solid matrix for 5 d at 15°C in the dark. Physiological indexes of unpriming and priming varieties were measured at (25±1)°C for 10 d under 0, 100 mmol·L^-1 NaCl solution respectively. Results showed that dry and fresh weights of stem and root were significantly increased, while content of MDA was decreased compared with seedlings without priming. Furthermore, the increase in the content of proline, chlorophyll and the activity of antioxidant enzymes and more osmotic substances accumulation were detected during the seedling growth period with priming under salt stress. It was found that Chlb was stable than Chla, carotenoid was stable than Chl (a+b). The results suggested that solid matrix priming could improve salt tolerance ability of tomato seedlings by increasing the activities of antioxidant enzymes, promoting osmotic substances synthesis in different organs, thereby decreasing membrane permeability and lipid peroxidation to protect the stability of cell membrane structure and photosynthetic apparatus.

Key words: tomato seedling; solid matrix priming; photosynthetic pigments; salt stress